Introduction: Rheumatoid arthritis (RA) is classified as an autoimmune inflammatory condition characterized by pain, swelling, and inflammation of the joints, along with stiffness which can reduce function and impair the overall quality of life. Rheumatoid arthritis initiated from chronic inflammatory disorder that can affect not only just the joints but it also damages a wide variety of body systems, including the skin, eyes, lungs, heart and blood vessels. Some patient cases in post- rheumatoid arthritis diagnosis develop cancer later. Moreover, a total of 138 cases of lung and prostate solid tumors were recorded within 12 months of RA diagnosis. Furthermore, those patients diagnosed with RA experienced cancer of greater severity than was the case for patients who did not have RA. Exercise may represent a novel means of mitigating the suffering of RA and cancer patients. A number of studies have sought to examine the application of exercise as a means of inhibiting tumorigenesis.

Methods: The effects of exercise interventions on serum microRNAs were investigated in pristane-induced arthritis (PIA) rat models. Twelve Sprague-Dawley male rats were divided into 4 groups including non-exercise without PIA (N-EX), non-exercise with PIA (N-EX + PIA), exercise without PIA (EX) and exercise with PIA (EX + PIA). Blood samples were collected at the end of the study period to analyze miRNA biomarkers and target cancer gene predictions.

Results: Four significant Rattus norvegicus (rno-microRNAs) may purpose as tumor suppressors were identified as potential target cancer gene candidate expressions within the 4 comparative interventional exercise groups. One rno-microRNA and target cancer gene candidate was up-regulated and 3 rno-microRNAs and their target cancer genes were down-regulated.