Ana Beatriz Pereira Riotinto
Afya Parnaíba, BrazilPresentation Title:
Bacterial analysis and culture of stethoscopes from physicians in Primary Health Care Units (PHC)
Abstract
Objective: To perform
bacterial analysis and culture on stethoscopes of physicians working in basic
health units in the city of XXX.
Methodology: Samples were collected from
stethoscopes used by physicians who provide care in Basic Health Units (UBS's)
in the city of XXX. First, the culture medium was prepared and then the
collected samples were seeded and the growth of microorganisms was observed in
an incubator, where the bacterial colonies developed in the culture medium.
Thus, the smear of the samples was obtained to perform Gram staining and then
the slides were analyzed under the microscope.
Results: Twelve strains were
isolated, of which eight were Gram-positive Cocci, two Gram-negative Bacilli
and two Gram-positive Bacilli. After the identification tests, it was found
that the Gram-positive cocci belonged to the genus Staphylococcus sp., while
the Gram-positive bacilli belonged to the genus Bacillus sp. and the
Gram negative bacilli were of the non-fermenting type, of the species
Achromobacter xylosoxidans.
Conclusion: The results of this study highlight the
presence of different bacterial genera, including Staphylococcus sp., Bacillus
sp. and Achromobacter, in stethoscopes of physicians in the UBS. These
bacteria, some of which are potentially pathogenic and associated with
nosocomial infections, reinforce the importance of adequate hygiene of medical
equipment as an essential measure to prevent healthcare associated infections
and ensure greater safety in the clinical environment.
Biography
Ana Beatriz Pereira Riotinto is a medical
student at the Parnaíba Valley Higher Education Institute (IESVAP), starting in
2022. She works in various areas of medical training, including research,
outreach, tutoring, and scientific production. She was a scholarship holder and
volunteer in scientific initiation projects in the areas of infectious
diseases, dermatology, and maternal and child health. She participated in
outreach initiatives focused on elderly health, child health, and health
education. She served as a tutor for the Teaching-Service-Community Integration
(IESC I and IV) and the Objective Structured Clinical Examination (OSCE). She
is currently president of the Academic League of Sports Medicine and Nutrology
(LAMEN). She has published articles in scientific journals and has written book
chapters. She has presented papers at national and international conferences
and symposia, receiving honorable mentions and awards, such as for the
"Corpo em Jogo" project (2025) and in the area of public health
(2023). During high school, she won a bronze medal at the Piauiense Chemistry
Olympiad. Ana Beatriz is interested in preventive medicine, research, public
health, and sports medicine, pursuing constant technical and scientific
development and a commitment to comprehensive and humanized healthcare
education.
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