Vitamin C is an essential water-soluble micronutrient recognized for its antioxidant properties, immune system support, and vital role in collagen synthesis. Despite its significance, the oral bioavailability of Vitamin C is limited due to the saturation of intestinal transporters and its rapid elimination via renal excretion. As an alternative, topical application has gained considerable attention, especially for promoting skin health. It supports dermal collagen production, offers photoprotection, and combats oxidative stress caused by environmental pollutants. To address the limitations of traditional delivery systems, West Bengal Chemical Industries Ltd. (WBCIL) developed an innovative liposomal Vitamin C formulation using non-GMO sunflower-derived phosphatidylcholine. This study evaluates the physicochemical characteristics, encapsulation efficiency, structural integrity, and stability of the liposomal product, as well as its potential for enhanced absorption. Advanced analytical techniques were employed for comprehensive characterization. High-Performance Liquid Chromatography (HPLC) demonstrated a high encapsulation efficiency of 91.98%. Fourier Transform Infrared Spectroscopy (FTIR) confirmed the presence of key functional groups, while X-Ray Photoelectron Spectroscopy (XPS) identified target surface elements, supporting the structural integrity of the formulation. Dynamic Light Scattering (DLS) analysis revealed a mean hydrodynamic particle size of approximately 184 nm, a zeta potential of -33.7 mV, and a polydispersity Index (PDI) of 0.334, indicating excellent electrostatic stability and uniform size distribution. Overall, the liposomal Vitamin C formulation exhibited high encapsulation efficiency (85–90%), nanoscale particle size, stable dispersion, and sustained release properties under physiological and thermal conditions. These attributes suggest enhanced dermal absorption and prolonged biological activity. The results affirm WBCIL’s liposomal formulation as a promising, bioavailable, and consumer-friendly alternative to conventional Vitamin C supplements, offering significant advantages in therapeutic, cosmetic, and preventive applications.